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Lecture Details :
Eukaryotic Gene Expression:Basics & Benefits by Prof.P N RANGARAJAN,Department of Biochemistry,IISC Bangalore. For more details on NPTEL visit http://nptel.iitm.ac.in
Course Description :
The objective of this course is to expose the viewer to basic aspects of regulation of gene expression in eukaryotes and explain the benefits that have accrued out of basic research in this area.
The course is organized such that the viewer will learn not only gain knowledge on the molecular mechanisms involved in eukaryotic gene regulation but also will appreciate the benefits that have accrued through the exploitation of knowledge gained from the basic research, primarily in the areas of disease diagnosis and their prevention, agriculture etc.
Through this course the instructor wishes to convey the message that knowledge of eukaryotic gene regulation is essential for understanding and appreciating a number of biotechnological applications in the areas of medicine and agriculture.
Contents:
CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS
Eukaryotic RNA polymerases and basal transcription factors
Diversity in core promoter elements
Diversity in general transcription factors
Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators
DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS
Transcription factors – DNA binding domains
Transcription factors – transcription activation domain
ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION
Role of chromatin in eukaryotic gene regulation
Role of histones in eukaryotic gene regulation
Role of DNA methylation in eukaryotic gene regulation
Chromatin remodelling & gene regulation
SYNTHESIS OF mRNA, rRNA & tRNA
mRNA processing – Role of RNA Pol II in mRNA capping and mRNA splicing
mRNA processing – Role of RNA Pol II in polyadenylation & mRNA editing
Regulation of RNA Pol I transcription
Regulation of RNA Pol III transcription
REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS
Signal Transduction Pathways - Introduction
Regulation of gene expression by cyclicAMP
Regulation of gene expression by second messengers other than cAMP
Regulation of gene expression by Protein Kinase C
Regulation of gene expression by Growth factors
Regulation of gene expression by cytokines
REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS
Regulation of gene expression by steroid hormones
Regulation of gene expression by type II nuclear receptors
Mechanism of transcriptional activation by nuclear receptors
REGULATION OF GENE EXPRESSION DURING DEVELOPMENT
Gene Regulation during Drosophila Development
Signal transduction pathways involved in embryonic development
Homeotic genes
Epigenetic regulation of gene expression during development
Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming
RECOMBINANT PROTEIN EXPRESSION SYSTEMS
Cloning and Expression vectors
Eukaryotic protein expression systems - I
Eukaryotic protein expression systems - II
Eukaryotic protein expression systems – III: Gene expression in mammalian cells using viral vectors
GENE THERAPY AND TRANSGENIC TECHNOLOGY
Human Gene Therapy
DNA vaccines
Transgenic animals
Transgenic plants
Knockout mice
RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSION
Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi)
Genomics & Proteomics
Metabolic Engineering & Synthetic Biology
Other Resources :
Syllabus | Handouts | Citation |
These lecture videos are delivered by IISc Bangalore, under the NPTEL program, lot of nptel video courses are available for learning online.
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