Analytical Technologies in Biotechnology

IIT Roorkee Course , Prof. Ashwani K Sharma

Lecture 1: Mod-01 Lec-01 Basic concepts in microscopy 1

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Lecture Details :

Analytical Technologies in Biotechnology by Dr. Ashwani K Sharma,Department of Biotechnology,IIT Roorkee.For more details on NPTEL visit

Course Description :

Basic concepts in microscopy - Dark-field and phase contrast microscopy - Differential interference contrast and polarization - Fluorescence and confocal microscopy - Transmission electron microscopy and scanning electron microscopy - Basic concepts - GM counting and Scintillation counting - Scintillation counting continued - Autoradiography and RIA - Safety aspects and applications - Introduction and Basic concepts in chromatography - Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography - Ion-exchange chromatography - Gel-filtration chromatography - Affinity chromatography - Gas-liquid chromatography - Basic concepts in electrophoresis - Horizontal and vertical gel electrophoresis - Native gel electrophoresis and SDS-PAGE - Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods

Electrophoresis of nucleic acids - Immunoelectrophoresis and capillary electrophoresis - Introduction and basic concepts - Types of centrifuges and analytical ultracentrifugation method - Separation methods in preparative ultracentrifuges - Types of rotors and care of rotors - Introduction and basic concepts - UV-Visible spectroscopy - Infrared and fluorescence spectroscopy - Circular dichroism (CD) spectroscopy - Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography - Atomic spectroscopy and mass spectrometry - Polymerase chain reaction(PCR) - DNA sequencing methods - Enzyme linked immunosorbent assay (ELISA)

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