Downstream Processing

IIT Madras Course , Prof. Mukesh Doble

Lecture 1: Mod-01 Lec-01 Introduction

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Lecture Details :

Downstream Processing by Prof. Mukesh Doble, Department of Biotechnology, IIT Madras. For more details on NPTEL visit http://nptel.iitm.ac.in

Course Description :

Contents:
Introduction and course contents - Bioprocess case studies/flow sheet - Characteristics of biomolecules and their differences - Various down stream process steps - Fundamental principles of obtaining the product from cell cultures intracellular vs. extracellular product - Cell disruption mechanical, enzymatic, and chemical methods - Pre-treatment strategies - Solid-liquid separation filtration and centrifugation

Adsorption - Liquid-liquid extraction - Membrane separation ultrafiltration and reverse osmosis, dialysis etc - Precipitation of proteins by different methods - Chromatograph principles, instruments and practice - Crystallization - Distillation - Drying - lyophilization - Stabilization of bioproducts - Utilities (Air, water, steam etc)

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