Eukaryotic Gene Expression

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CIS-ACTING ELEMENTS AND TRANS-ACTING FACTORS:Eukaryotic RNA polymerases and basal transcription factors – Diversity in core promoter elements – Diversity in general transcription factors – Proximal & Distal Promoter Elements, Enhancers and Silencers, Gene-specific Regulators,DOMAIN STRUCTURE OF EUKARYOTIC TRANSCRIPTION FACTORS:Transcription factors – DNA binding domains – Transcription factors – transcription activation domain,ROLE OF CHROMATIN IN EUKARYOTIC GENE REGULATION:Role of chromatin in eukaryotic gene regulation – Role of histones in eukaryotic gene regulation – Role of DNA methylation in eukaryotic gene regulation – Chromatin remodelling & gene regulation,SYNTHESIS OF mRNA, rRNA & tRNA:mRNA processing – Role of RNA Pol II in mRNA capping and mRNA splicing – mRNA processing – Role of RNA Pol II in polyadenylation & mRNA editing – Regulation of RNA Pol I transcription – Regulation of RNA Pol III transcription,REGULATION OF GENE EXPRESSION VIA CELL SURFACE RECEPTORS:Signal Transduction Pathways – Introduction – Regulation of gene expression by cyclicAMP – Regulation of gene expression by second messengers other than cAMP – Regulation of gene expression by Protein Kinase C – Regulation of gene expression by Growth factors – Regulation of gene expression by cytokines,REGULATION OF GENE EXPRESSION BY INTRACELLULAR RECEPTORS:Regulation of gene expression by steroid hormones – Regulation of gene expression by type II nuclear receptors – Mechanism of transcriptional activation by nuclear receptors

REGULATION OF GENE EXPRESSION DURING DEVELOPMENT:Gene Regulation during Drosophila Development – Signal transduction pathways involved in embryonic development – Homeotic genes – Epigenetic regulation of gene expression during development – Embryonic stem cells and Transcription factor-mediated epigenetic – reprogramming,RECOMBINANT PROTEIN EXPRESSION SYSTEMS:Cloning and Expression vectors – Eukaryotic protein expression systems – Gene expression in mammalian cells using viral vectors,GENE THERAPY AND TRANSGENIC TECHNOLOGY:Human Gene Therapy – DNA vaccines – Transgenic animals – Transgenic plants – Knockout mice,RECENT ADVANCES IN EUKARYOTIC GENE EXPRESSION:Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi) – Genomics & Proteomics – Metabolic Engineering & Synthetic Biology

Course Curriculum

Eukaryotic RNA polymerases and basal transcription factors Details 53:36
Diversity in core promoter elements Details 53:51
Diversity in general transcription factors I Details 53:5
Proximal & Distal Promoter Elements,Enhancers and Silencers, Gene-specific Regulators Details 59:2
Transcription factors : DNA binding domains Details 55:30
Transcription factors: transcription activation domains I Details 50:52
Role of chromatin in eukaryotic gene regulation Details 52:44
Role of histones in eukaryotic gene regulation I Details 54:15
Role of DNA methylation in eukaryotic gene regulation I Details 52:6
Chromatin remodelling & gene regulation Details 51:58
Co-transcriptional and post-transcriptional modifications of pre messenger RNA-I Details 53:26
Co-transcriptional and post-transcriptional modifications of pre messenger RNA-II Details 58:16
Regulation of RNA Pol I transcription Details 55:7
Regulation of tRNA and 5s rRNA synthesis by RNA Polymerase III Details 56:34
Signal Transduction Pathways – Introduction Details 55:17
Regulation of gene expression by cyclicAMP Details 57:30
Regulation of gene expression by second messengers other than cAMP Details 52:15
Regulation of gene expression by Protein Kinase C Details 57:49
Regulation of gene expression by Growth factors Details 58:20
Regulation of gene expression by cytokines Details 56:5
Regulation of gene expression by steroid hormones Details 58:3
Regulation of gene expression by type II nuclear receptors Details 57:55
Mechanism of transcriptional activation by nuclear receptors Details 57:55
Gene Regulation during Drosophila Development Details 56:30
Signal transduction pathways involved in embryonic development Details 55:29
Homeotic genes Details 57:32
Epigenetic regulation of gene expression during development Details 57:13
Embryonic stem cells and Transcription factor-mediated epigenetic reprogramming Details 57:53
Cloning and Expression vectors Details 57:42
Eukaryotic protein expression systems – I Details 56:5
Eukaryotic protein expression systems – II Details 57:13
Eukaryotic protein expression systems-III Details 56:56
Human Gene Therapy Details 57:34
DNA vaccines Details 0:57
Transgenic animals Details 55:36
Transgenic plants Details 58:40
Knockout mice Details 58:9
Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi) Details 58:10
Genomics & Proteomics Details 58:3
Metabolic Engineering & Synthetic Biology Details 57:11

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