Frontiers of Biomedical Engineering
Yale,, Spring 2008 , Prof. W. Mark Saltzman
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Updated On 02 Feb, 19
Biomedical Engineering, Genetic Engineering, Cell Culture Engineering, Bio-molecular Engineering, Cardiovascular Physiology, Renal Physiology, Biomechanics and Orthopedics, Bioimaging, Tissue Engineering.
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Professor Saltzman continues his presentation on DNA technology by discussing control of gene expression using two methods of RNA silencing anti-sense therapy and RNA interference. Molecular cloning techniques to mass-produce proteins using plasmid, restriction enzymes, ligase, and antibiotic selection in bacteria are discussed. Steps and molecules involved in polymerase chain reaction are also described. Professor Saltzman explains how to detect mutations in genomic DNA, such as in sickle cell anemia patients, by gel electrophoresis and Southern blotting. Finally, he gives an example of inducing and controlling insulin expression in large animals by cloning into their genome the insulin gene with the lac promoter.
Sep 12, 2018
Excellent course helped me understand topic that i couldn't while attendinfg my college.
March 29, 2019
Great course. Thank you very much.