Basic concepts in microscopy – Dark-field and phase contrast microscopy – Differential interference contrast and polarization – Fluorescence and confocal microscopy – Transmission electron microscopy and scanning electron microscopy – Basic concepts – GM counting and Scintillation counting – Scintillation counting continued – Autoradiography and RIA – Safety aspects and applications – Introduction and Basic concepts in chromatography – Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography – Ion-exchange chromatography – Gel-filtration chromatography – Affinity chromatography – Gas-liquid chromatography – Basic concepts in electrophoresis – Horizontal and vertical gel electrophoresis – Native gel electrophoresis and SDS-PAGE – Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods

Electrophoresis of nucleic acids – Immunoelectrophoresis and capillary electrophoresis – Introduction and basic concepts – Types of centrifuges and analytical ultracentrifugation method – Separation methods in preparative ultracentrifuges – Types of rotors and care of rotors – Introduction and basic concepts – UV-Visible spectroscopy – Infrared and fluorescence spectroscopy – Circular dichroism (CD) spectroscopy – Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography – Atomic spectroscopy and mass spectrometry – Polymerase chain reaction(PCR) – DNA sequencing methods – Enzyme linked immunosorbent assay (ELISA)

Other Resources

Course Curriculum

Mod-01 Lec-01 Basic concepts in microscopy 1 Details 48:17
Mod-01 Lec-02 Basic concepts in microscopy 2 Details 43:23
Mod-01 Lec-03 Dark-field and phase contrast microscopy Details 45:49
Mod-01 Lec-04 Differential interference contrast and polarization Details 0:44
Mod-01 Lec-05 Fluorescence and confocal microscopy Details 41:7
Mod-01 Lec-06 Transmission electron microscopy Details 46:27
Mod-01 Lec-07 Transmission electron microscopy cont. and scanning electron microscopy Details 43:31
Mod-02 Lec-01 Basic concepts 1 Details 43:5
Mod-02 Lec-02 Basic concepts 2 Details 41:47
Mod-02 Lec-03 GM counting and Scintillation counting Details 47:9
Mod-02 Lec-04 Scintillation counting continued Details 39:54
Mod-02 Lec-05 Autoradiography and RIA Details 54:19
Mod-02 Lec-06 Safety aspects and applications Details 49:45
Mod-03 Lec-01 Introduction and Basic concepts in chromatography 1 Details 42:21
Mod-03 Lec-02 Basic concepts in chromatography 2 Details 47:48
Mod-03 Lec-03 Low-pressure liquid chromatography (LPLC) and high performance liquid chromatography Details 52:48
Mod-03 Lec-04 Ion-exchange chromatography Details 48:39
Mod-03 Lec-05 Gel-filtration chromatography Details 48:57
Mod-03 Lec-06 Affinity chromatography Details 41:5
Mod-03 Lec-07 Gas-liquid chromatography Details 50:43
Mod-04 Lec-01 Basic concepts in electrophoresis Details 47:25
Mod-04 Lec-02 Horizontal and vertical gel electrophoresis Details 46:3
Mod-04 Lec-03 Native gel electrophoresis and SDS-PAGE Details 47:52
Mod-04 Lec-04 Isoelectric focusing (IEF), 2-D gel electrophoresis and protein detection methods Details 52:50
Mod-04 Lec-05 Electrophoresis of nucleic acids Details 50:41
Mod-04 Lec-06 Immunoelectrophoresis and capillary electrophoresis Details 54:8
Mod-05 Lec-01 Introduction and basic concepts 1 Details 46:17
Mod-05 Lec-02 Basic concepts-2 Details 48:17
Mod-05 Lec-03 Types of centrifuges and analytical ultracentrifugation method Details 55:3
Mod-05 Lec-04 Separation methods in preparative ultracentrifuges Details 51:14
Mod-05 Lec-05 Types of rotors Details 48:16
Mod-05 Lec-06 Types of rotors cont. and care of rotors Details 47:58
Mod-06 Lec-01 Introduction and basic concepts Details 50:57
Mod-06 Lec-02 UV-Visible spectroscopy Details 49:58
Mod-06 Lec-03 Infrared and fluorescence spectroscopy Details 54:43
Mod-06 Lec-04 Circular dichroism (CD) spectroscopy Details 46:55
Mod-06 Lec-05 Nuclear magnetic resonance (NMR) spectroscopy and X-ray crystallography Details 1:4:26
Mod-06 Lec-06 Atomic spectroscopy and mass spectrometry Details 51:22
Mod-07 Lec-01 Polymerase chain reaction(PCR) Details 52:20
Mod-07 Lec-02 DNA sequencing methods Details 1:45
Mod-07 Lec-03 Enzyme linked immunosorbent assay (ELISA) Details 44:13

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