Contents:
Introduction and course contents – Bioprocess case studies/flow sheet – Characteristics of biomolecules and their differences – Various down stream process steps – Fundamental principles of obtaining the product from cell cultures – intracellular vs. extracellular product – Cell disruption – mechanical, enzymatic, and chemical methods – Pre-treatment strategies – Solid-liquid separation – filtration and centrifugation

Adsorption – Liquid-liquid extraction – Membrane separation – ultrafiltration and reverse osmosis, dialysis etc – Precipitation of proteins by different methods – Chromatograph – principles, instruments and practice – Crystallization – Distillation – Drying – lyophilization – Stabilization of bioproducts – Utilities (Air, water, steam etc)

Other Resources

Course Curriculum

Mod-01 Lec-01 Introduction Details 54:43
Mod-01 Lec-02 Mass balance, Heat Balance, flow sheet Details 51:33
Mod-01 Lec-03 Costing Details 53:10
Mod-01 Lec-06 Cell Breakage Details 52:22
Mod-01 Lec-08 Solid Liquid Separation Details 51:51
Mod-01 Lec-09 Solid Liquid Separation (continued) Details 53:56
Mod-01 Lec-10 Solid Liquid separation-problems Details 52:11
Mod-01 Lec-11 Pre-treatment and Filters Details 53:6
Mod-01 Lec-12 Adsorption Details 53:45
Mod-01 Lec-13 Adsorption (continued) Details 54:35
Mod-01 Lec-14 Adsorption (continued) Details 51:54
Mod-01 Lec-16 Liquid-Liquid Extraction Details 51:35
Mod-01 Lec-17 Liquid-Liquid extraction (continued) Details 50:51
Mod-01 Lec-18 Liquid-Liquid extraction (continued) Details 33:59
Mod-01 Lec-19 Liquid-Liquid extraction (continued) Details 51:20
Mod-01 Lec-20 Reversed micellar and aqueous two phase extraction Details 50:51
Mod-01 Lec-21 Membranes Details 51:44
Mod-01 Lec-22 Membranes (continued) Details 48:32
Mod-01 Lec-23 Membranes (continued) Details 49:47
Mod-01 Lec-24 Membranes (continued) Details 51:58
Mod-01 Lec-25 Precipitation Details 50:57
Mod-01 Lec-26 Chromatography Details 52:52
Mod-01 Lec-27 Chromatography (continued) Details 51:25
Mod-01 Lec-28 Chromatography (continued) Details 51:47
Mod-01 Lec-29 Chromatography (continued) Details 50:50
Mod-01 Lec-30 Chromatography (continued) Details 50:19
Mod-01 Lec-31 Chromatography (continued) Details 51:48
Mod-01 Lec-32 Chromatography (continued) Details 47:27
Mod-01 Lec-33 HPLC Details 51:51
Mod-01 Lec-34 HPLC Details 50:20
Mod-01 Lec-37 Drying and Distillation Details 50:57
Mod-01 Lec-38 Stabilisation, Utilities and other auxiliary processes and absorption Details 50:59
Mod-01 Lec-39 Absorption, Electrophoresis / SDS PAGE Details 48:15
Mod-01 Lec-40 Future trends, Summary of the course Details 50:8
Mod-01 Lec15 Adsorption (continued) Details 51:36
Mod-01 Lec-04 Costing (continued), Physical and chemical principles in Downs stream Details 56:27
Mod-01 Lec-05 Problems in Mass balance, flow sheet Details 50:23
Mod-01 Lec-07 Cell Breakage (continued) Details 44:40
Mod-01 Lec-35 Crystallisation Details 52:9
Mod-01 Lec-36 Drying Details 53:1

This course is delivered by NPTEL, is part of IIT Madras online courses.

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