Downstream Processing

Overview

Contents:
Introduction and course contents - Bioprocess case studies/flow sheet - Characteristics of biomolecules and their differences - Various down stream process steps - Fundamental principles of obtaining the product from cell cultures intracellular vs. extracellular product - Cell disruption mechanical, enzymatic, and chemical methods - Pre-treatment strategies - Solid-liquid separation filtration and centrifugation

Adsorption - Liquid-liquid extraction - Membrane separation ultrafiltration and reverse osmosis, dialysis etc - Precipitation of proteins by different methods - Chromatograph principles, instruments and practice - Crystallization - Distillation - Drying - lyophilization - Stabilization of bioproducts - Utilities (Air, water, steam etc)