Proteomics: Principles and Techniques

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Introduction to Proteomics Course – Central Dogma: Basics of DNA, RNA, Proteins – Genomics and Transcriptomics: Why proteomics – Proteins: Amino acids and structural levels of proteins – Proteins: Folding and misfolding – Protein Purification and Peptide Isolation using Chromatography – Enzymes: Basic concepts, Catalytic and Regulatory strategies – Proteomics and Systems Biology – Sample preparation for proteomics applications – Sample preparation for proteomics applications: Serum and bacterial proteome – Sample preparation for proteomics applications – Gel-based Proteomics Two-dimensional electrophoresis Workflow – Two-dimensional electrophoresis: Image processing and data analysis – Two-dimensional difference gel electrophoresis (2D DIGE) – Difference gel electrophoresis (continued) Discussion and data analysis – Applications of two dimensional electrophoresis – Applications of 2-DE and DIGE – Fundamentals of mass spectrometry – Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF) – Liquid chromatography-Mass spectrometry (LC-MS/MS) – Hybrid-MS/MS Configurations – Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell – Culture(SILAC)

Quantitative Proteomics: iTRAQ and TMT – Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays – Microarray workflow: Label-based detection techniques – Microarray related concepts: Recombinational cloning Cell-free expression – Cell-free synthesis based protein microarrays – Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array – Microarray work-flow: Image scanning and processing – Microarray work-flow: Data analysis – Applications of protein microarrays – Applications of cell free protein microarrays I – Label-free techniques: SPR and SPRi – Label-free techniques: SPRi, Ellipsometry, Interference – Surface Plasmon Resonance: Biacore SPR and data analysis – Nanotechniques in proteomics – Detection System: Diffraction-based biosensors – Proteomics: Advances and Challenges

Course Curriculum

Introduction to Proteomics Course Details 1:3:26
Central Dogma: Basics of DNA, RNA, Proteins Details 48:57
Genomics and Transcriptomics: Why proteomics? Details 45:42
Proteins: Amino acids and structural levels of proteins Details 52:53
Proteins: Folding and misfolding Details 47:45
Protein Purification and Peptide Isolation using Chromatography Details 38:39
Enzymes: Basic concepts, Catalytic and Regulatory strategies Details 56:9
Proteomics and Systems Biology Details 1:12:45
Sample preparation for proteomics applications Details 48:52
Sample preparation for proteomics applications: Serum and bacterial proteome I Details 55:24
Sample preparation for proteomics applications II Details 51:26
Gelbased Proteomics Details 55:39
Gel-based Proteomics Two-dimensional electrophoresis I Details 49:5
Gel-based Proteomics Two-dimensional electrophoresis Workflow II Details 57:36
Two-dimensional electrophoresis: Image processing and data analysis Details 1:5:57
Two-dimensional difference gel electrophoresis (2D DIGE) I Details 45:19
Difference gel electrophoresis (continued) Discussion and data analysis Details 45:14
Applications of two dimensional electrophoresis Details 44:34
Applications of 2-DE and DIGE I Details 45:24
Fundamentals of mass spectrometry Details 47:5
Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF) Details 55:1
Liquid chromatography-Mass spectrometry (LC-MS/MS) Details 51:41
Hybrid-MS/MS Configurations Details 41:27
Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell Culture(SILAC) Details 50:56
Quantitative Proteomics: iTRAQ and TMT I Details 43:49
Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays Details 53:11
Microarray workflow: Label-based detection techniques Details 44:20
Microarray related concepts: Recombinational cloning Cell-free expression Details 56:11
Cell-free synthesis based protein microarrays Details 56:23
Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array Details 59:14
Microarray work-flow: Image scanning and processing Details 55:48
Microarray work-flow: Data analysis I Details 1:2:11
Applications of protein microarrays Details 53:13
Applications of cell free protein microarrays I Details 59:10
Label-free techniques: SPR and SPRi Details 48:25
Label-free techniques: SPRi, Ellipsometry, Interference I Details 41:34
Surface Plasmon Resonance: Biacore SPR and data analysis Details 43:36
Nanotechniques in proteomics Details 52:9
Detection System: Diffraction-based biosensors I Details 52:57
Proteomics: Advances and Challenges Details 1:7:38

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