Getting Started in Cryo-EM (cryo-electron microscopy)

Overview

This course covers the fundamental principles underlying cryo-electron microscopy starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography