Introduction to mechanobiology

COURSE LAYOUT

Week 1
Lecture 1: Need to study Mechanobiology
Lecture 2: Cell as a Tent, individual components
Lecture 3: Cell-ECM crosstalk
Lecture 4: ECM proteins: Collagen
Lecture 5: Measuring properties of collagen networks

Week 2
Lecture 6:   Properties of collagen networks
Lecture 7:   Rheology
Lecture 8:   Rheology of biopolymer networks
Lecture 9:   Atomic Force Microscopy (AFM)
Lecture 10: Design of protein constructs for AFM

Week 3
Lecture 11: Protein unfolding using AFM
Lecture 12: Protein unfolding using AFM
Lecture 13: Focal adhesions: focal adhesion proteins
Lecture 14: Focal adhesion organization
Lecture 15: Focal adhesions: role of forces

Week 4
Lecture 16: Cytoskeleton: Actin
Lecture 17: Force-velocity relationships of actin networks
Lecture 18: Mesenchymal cell migration
Lecture 19: Actin dynamics during mesenchymal migration
Lecture 20: Actin dynamics during mesenchymal migration

Week 5
Lecture 21: Adhesion Independent Migration
Lecture 22: Adhesion Independent & Collective Cell Migration
Lecture 23: Collective Cell Migration
Lecture 24: Mechanobiology of Stem Cell Fate - I
Lecture 25: Mechanobiology of Stem Cell Fate - II

Week 6
Lecture 26: Mechanobiology of Stem Cell Fate - III
Lecture 27: Mechanobiology of Diseases: Cancer I
Lecture 28: Mechanobiology of Diseases: Cancer II
Lecture 29: Mechanobiology of Diseases: Cancer III
Lecture 30: Mechanobiology of Diseases: Atherosclerosis & Hypertension

Week 7
Lecture 31: Mechanobiology of Diseases: Muscular Dystrophy
Lecture 32: Nuclear Mechanotransduction: LINC complex
Lecture 33: Nuclear Mechanotransduction: LINC complex in cell migration
Lecture 34: Nuclear Mechanotransduction: Gene regulation
Lecture 35: Mechanical Forces & DNA damage

Week 8
Lecture 36: Techniques in Mechanobiology: Hydrogels
Lecture 37: Techniques in Mechanobiology: AFM
Lecture 38: Techniques in Mechanobiology: Traction Force Microscopy, Trypson Deadhesion
                    & Laser Ablation
Lecture 39: Techniques in Mechanobiology: Microfabrication
Lecture 40: Techniques in Mechanobiology: FRE