Introduction to proteomics

IIT Bombay, , Prof. Prof. Sanjeeva Srivastava

Updated On 02 Feb, 19

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Lecture 1: Lecture 1 : Introduction to amino acids

4.1 ( 11 )

Lecture Details

Course Details

COURSE LAYOUT

Week 1 : Basics of Proteins and Proteomics

Lecture 1 : Introduction to amino acids
Lecture 2 : Introduction to Proteins
Lecture 3 : Protein folding & misfolding
Lecture 4 : Introduction to Proteomics
Lecture 5 : Lab session Protein-protein interaction using label-free biosensors

Week 2 : Gel-based proteomics

Lecture 6: Sample preparation and pre-analytical factors
Lecture 7 : Sample preparation: Pre-analytical factors (contd.)
Lecture 8 : Sample preparation: Protein extraction and quantification
Lecture 9 : One-dimensional electrophoresis
Lecture 10 : Introduction to 2-DE

Week 3 : Two-dimensional gel electrophoresis (2-DE)

Lecture 11 : 2-DE: Second dimension, staining & destaining
Lecture 12 : 2-DE: Gel analysis
Lecture 13 : 2-DE Applications
Lecture 14 : 2-DE Applications (contd.) & Challenges
Lecture 15 : Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis

Week 4 : Difference in gel electrophoresis (DIGE) & Systems Biology

Lecture 16 : 2D-DIGE: Basics
Lecture 17 : 2D-DIGE: Data analysis
Lecture 18 : 2D-DIGE: Applications
Lecture 19 : Systems biology and proteomics I
Lecture 20 : Systems biology and proteomics - II

Week 5 : Basics of mass spectrometry

Lecture 21 : Fundamentals of mass spectrometry
Lecture 22 : Chromatography technologies
Lecture 23 : Liquid chromatography
Lecture 24 : Mass spectrometry: Ionization sources
Lecture 25 : Mass spectrometry: Mass analyzers

Week 6 : Basics of mass spectrometry and sample preparation

Lecture 26 : MALDI sample preparation and analysis
Lecture 27 : Hybrid mass spectrometry configurations
Lecture 28 : Lab session - Demonstration of Q-TOF MS technology
Lecture 29 : In-gel & in-solution digestion
Lecture 30 : Lab session - Sample preparation: tissue sample preservation technology

Week 7 : Quantitative proteomics

Lecture 31 : Introduction to quantitative proteomics
Lecture 32 : SILAC: In vivo labelling
Lecture 33 : iTRAQ: In vitro labelling
Lecture 34 : TMT: In vitro labelling
Lecture 35 : Quantitative proteomics data analysis

Week 8 :

Advancement in Proteomics

Lecture 36 : Proteomics applications
Lecture 37 : Challenges in proteomics
Lecture 38 : OMICS and translational research
Lecture 39 : Lab session Targeted proteomics using triple quadrupole mass spectrometry
Lecture 40 : Lab session Targeted proteomics: multiple reaction monitoring