Proteomics: Principles and Techniques

IIT Bombay Course , Prof. Sanjeeva Srivastava

53 students enrolled

Overview

Introduction to Proteomics Course - Central Dogma: Basics of DNA, RNA, Proteins - Genomics and Transcriptomics: Why proteomics - Proteins: Amino acids and structural levels of proteins - Proteins: Folding and misfolding - Protein Purification and Peptide Isolation using Chromatography - Enzymes: Basic concepts, Catalytic and Regulatory strategies - Proteomics and Systems Biology - Sample preparation for proteomics applications - Sample preparation for proteomics applications: Serum and bacterial proteome - Sample preparation for proteomics applications - Gel-based Proteomics Two-dimensional electrophoresis Workflow - Two-dimensional electrophoresis: Image processing and data analysis - Two-dimensional difference gel electrophoresis (2D DIGE) - Difference gel electrophoresis (continued) Discussion and data analysis - Applications of two dimensional electrophoresis - Applications of 2-DE and DIGE - Fundamentals of mass spectrometry - Matrix assisted laser desorption/ionization-Time of Flight (MALDI-TOF) - Liquid chromatography-Mass spectrometry (LC-MS/MS) - Hybrid-MS/MS Configurations - Quantitative Proteomics: Stable Isotope Labeling by Amino Acids in Cell - Culture(SILAC)

Quantitative Proteomics: iTRAQ and TMT - Interactomics: Yeast Two-Hybrid Immunoprecipitation Protein microarrays - Microarray workflow: Label-based detection techniques - Microarray related concepts: Recombinational cloning Cell-free expression - Cell-free synthesis based protein microarrays - Generating Protein Microarrays: Focus on Nucleic Acid Programmable Protein Array - Microarray work-flow: Image scanning and processing - Microarray work-flow: Data analysis - Applications of protein microarrays - Applications of cell free protein microarrays I - Label-free techniques: SPR and SPRi - Label-free techniques: SPRi, Ellipsometry, Interference - Surface Plasmon Resonance: Biacore SPR and data analysis - Nanotechniques in proteomics - Detection System: Diffraction-based biosensors - Proteomics: Advances and Challenges

Lecture 22: Liquid chromatography-Mass spectrometry (LC-MSMS)

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        Lecture Details

        Proteomics Principles and Techniques by Prof. Sanjeeva Srivastava, Department of Biotechnology, IIT Bombay. For more details on NPTEL visit httpnptel.iitm.ac.in

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